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eclipse ts2r fluorescence microscope  (Nikon)


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    Nikon eclipse ts2r fluorescence microscope
    Eclipse Ts2r Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2r+microscope/ECLIPSE+Ts2R/pmc13657077-80-14-13
    Average 99 stars, based on 1619 article reviews
    eclipse ts2r fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Discovery of inhibitors of the cancer-promoting phosphatase PRL-3 and their evaluation in intestinal organoids.
    Article Snippet: .. After 24 h incubation at 37 ◦C images of organoid phenotype were obtained by Eclipse Ts2R microscope (Plan Fluor 4×/0.13 ND, 16.5 WD, cover slide thickness 1.2; Nikon). .. For inhibitor assessment around 50 crypts of SI organoids in 10 μl diluted Matrigel (Growth Factor Reduced Basement Membrane Matrix, phenol red-free; Corning cat. no. 356238; diluted with PBS to 3 mg/mL protein concentration) were seeded per well in a sterile 96-well transparent plate (Greiner).

    Microscopy:

    Article Title: Discovery of inhibitors of the cancer-promoting phosphatase PRL-3 and their evaluation in intestinal organoids.
    Article Snippet: .. After 24 h incubation at 37 ◦C images of organoid phenotype were obtained by Eclipse Ts2R microscope (Plan Fluor 4×/0.13 ND, 16.5 WD, cover slide thickness 1.2; Nikon). .. For inhibitor assessment around 50 crypts of SI organoids in 10 μl diluted Matrigel (Growth Factor Reduced Basement Membrane Matrix, phenol red-free; Corning cat. no. 356238; diluted with PBS to 3 mg/mL protein concentration) were seeded per well in a sterile 96-well transparent plate (Greiner).

    Article Title: Multiplexed CRISPR/Cas9 Editing of Tumor Suppressor Genes Recapitulates Molecular and Morphological Features of High-Risk Endometrial Cancer
    Article Snippet: Clusters were diluted in basal medium supplemented with 2% dextran-coated charcoal-stripped serum (DCC) (A33821-01, Gibco) and plated into 96-well plates (black with micro-clear bottom) (655077, Greiner Bio One). .. When required, phase-contrast or fluorescence images were taken with Eclipse Ts2R microscope (Nikon), and quantification of GFP-positive cells was performed using Fiji software (version 2.16). ..

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds
    Article Snippet: .. The biodome was then placed in a humidified incubator at 37 °C and 5% CO 2 for 30 min. After 30 min, the Biodome was imaged using Eclipse Ts2R microscope (Nikon, Minato City, Tokyo, Japan) with a Coolsnap Dyno CCD (Photometrics, Tucson, Arizona, USA) and 10x objective. .. A FITC filter (EX: 482 nm, EM: 536 nm; Nikon) was used to excite the live stain (Hoechst 33342) while a DAPI filter (EX: 390 nm, EM: 475 nm; Nikon) was used to visualize the dead stain.

    Article Title: An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds.
    Article Snippet: .. After 30min, the Biodome was imaged using Eclipse Ts2R microscope (Nikon, Minato City, Tokyo, Japan) with a Coolsnap Dyno CCD (Photometrics, Tucson, Arizona, USA) and 10x objective. .. A FITC filter (EX: 482 nm, EM: Communications Engineering | (2025) 4:162 10 536 nm; Nikon) was used to excite the live stain (Hoechst 33342) while a DAPIfilter (EX: 390 nm,EM: 475 nm;Nikon)wasused to visualize thedead stain.

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction.
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Fluorescence:

    Article Title: Multiplexed CRISPR/Cas9 Editing of Tumor Suppressor Genes Recapitulates Molecular and Morphological Features of High-Risk Endometrial Cancer
    Article Snippet: Clusters were diluted in basal medium supplemented with 2% dextran-coated charcoal-stripped serum (DCC) (A33821-01, Gibco) and plated into 96-well plates (black with micro-clear bottom) (655077, Greiner Bio One). .. When required, phase-contrast or fluorescence images were taken with Eclipse Ts2R microscope (Nikon), and quantification of GFP-positive cells was performed using Fiji software (version 2.16). ..

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction.
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Software:

    Article Title: Multiplexed CRISPR/Cas9 Editing of Tumor Suppressor Genes Recapitulates Molecular and Morphological Features of High-Risk Endometrial Cancer
    Article Snippet: Clusters were diluted in basal medium supplemented with 2% dextran-coated charcoal-stripped serum (DCC) (A33821-01, Gibco) and plated into 96-well plates (black with micro-clear bottom) (655077, Greiner Bio One). .. When required, phase-contrast or fluorescence images were taken with Eclipse Ts2R microscope (Nikon), and quantification of GFP-positive cells was performed using Fiji software (version 2.16). ..

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction.
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Article Title: Irisin Increases Sirtuin 1 to Improve Glucocorticoid-Induced Sarcopenia and Mitochondrial Dysfunction
    Article Snippet: .. Fluorescence images were acquired with a Eclipse Ts2R microscope (Nikon, Yokohama, Japan) and processed using the NIS-Elements software (v4.20). ..

    Cell Culture:

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Staining:

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).



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